Overview

  • Product nameAnti-TRF2 antibody
    See all TRF2 primary antibodies
  • Description
    Goat polyclonal to TRF2
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a sequence in Human TFR2.

  • Positive control
    • Human testis tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab111118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionBinds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
  • Tissue specificityUbiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
  • Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
  • DomainThe TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Telomeric DNA binding protein antibody
    • Telomeric DNA-binding protein antibody
    • Telomeric repeat binding factor 2 antibody
    • Telomeric repeat binding protein 2 antibody
    • Telomeric repeat-binding factor 2 antibody
    • TERF 2 antibody
    • Terf2 antibody
    • TERF2_HUMAN antibody
    • TRBF 2 antibody
    • TRBF2 antibody
    • TRF 2 antibody
    • TTAGGG repeat binding factor 2 antibody
    • TTAGGG repeat-binding factor 2 antibody
    see all

Anti-TRF2 antibody images

  • ab111118, at 3.75µg/ml, staining TRF2 in formalin-fixed, paraffin-embedded Human Testis tissue by Immunohistochemistry.

References for Anti-TRF2 antibody (ab111118)

ab111118 has not yet been referenced specifically in any publications.

Product Wall

I have heard back from the lab regarding the antibodies ab13579, ab14402 & ab111118:


Ab13579 : Full-length recombinant human TRF2 protein was used as immunogen to generate the antibody.


We have not mapped the epitope...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"