Overview

  • Product nameAnti-TRF2 antibody
    See all TRF2 primary antibodies
  • Description
    Rabbit polyclonal to TRF2
  • SpecificityThis antibody detects bands of 65 and 69 kDa which correspond to TRF2.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from residues 250 - 350 of Human TRF2.

    .

  • Positive control
    • HeLa nuclear extract; HeLa whole cell lysate; HeLa nuclear lysate; HeLa whole cell lysate;
  • General notes


    Human telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex required for the protection and replication of chromosome ends. TRF2 is ubiquitously expressed, bound specifically to duplex TTAGGG repeats in vitro, and localized to all human telomeres in metaphase chromosomes. It is expressed as 2 proteins of 65 and 69 kD. Affinity-purified antibodies specific for anti-TRF2 labeled the telomeres of intact human chromosomes, strengthening the correlation between occurrence of telobox and telomere-repeat recognition in vivo. TRF2 is likely to bind to double-stranded telomeric DNA as a homodimer.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesHuman telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex required for the protection and replication of chromosome ends. TRF2 is ubiquitously expressed, bound specifically to duplex TTAGGG repeats in vitro, and localized to all human telomeres in metaphase chromosomes. It is expressed as 2 proteins of 65 and 69 kD. Affinity-purified antibodies specific for anti-TRF2 labeled the telomeres of intact human chromosomes, strengthening the correlation between occurrence of telobox and telomere-repeat recognition in vivo. TRF2 is likely to bind to double-stranded telomeric DNA as a homodimer.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Detects a band of approximately 65, 69 kDa (predicted molecular weight: 55.5 kDa).

Target

  • FunctionBinds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
  • Tissue specificityUbiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
  • Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
  • DomainThe TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Telomeric DNA binding protein antibody
    • Telomeric DNA-binding protein antibody
    • Telomeric repeat binding factor 2 antibody
    • Telomeric repeat binding protein 2 antibody
    • Telomeric repeat-binding factor 2 antibody
    • TERF 2 antibody
    • Terf2 antibody
    • TERF2_HUMAN antibody
    • TRBF 2 antibody
    • TRBF2 antibody
    • TRF 2 antibody
    • TTAGGG repeat binding factor 2 antibody
    • TTAGGG repeat-binding factor 2 antibody
    see all

Anti-TRF2 antibody images

  • All lanes : Anti-TRF2 antibody (ab4182) at 1 µg/ml

    Lane 1 : HeLa nuclear extract
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HeLa nuclear lysate with Human TRF2 peptide (ab13765) at 1 µg/ml
    Lane 4 : HeLa whole cell lysate with Human TRF2 peptide (ab13765) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Predicted band size : 55.5 kDa
  • ICC/IF image of ab4182 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab4182, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab4182 staining TRF2 in human Breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton-x100 and blocking with 2% serum was performed for 30 minutes at 370C. Samples were incubated with primary antibody at 1/100 dilution in PBS for 2 hours at 37°C. An Alexa Fluor® 594 conjugated goat monoclonal to rabbit IgG was used at 1/75 dilution as secondary antibody.

    See Abreview

References for Anti-TRF2 antibody (ab4182)

This product has been referenced in:
  • Mao Z  et al. TRF2 is required for repair of nontelomeric DNA double-strand breaks by homologous recombination. Proc Natl Acad Sci U S A 104:13068-73 (2007). Read more (PubMed: 17670947) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization Yes - Triton 0,1%
Fixative Paraformaldehyde
Username

Dr. Nicole Wagner

Verified customer

Submitted Dec 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Breast carcinoma)
Specification Breast carcinoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Aug 23 2009

Thank you for your message. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. However, since you used it on species which ab4182 has not previously been tested on - mou...

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