Overview

  • Product name
    Anti-TRF2 antibody [EPR3517(2)]
    See all TRF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3517(2)] to TRF2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human TRF2.

  • Positive control
    • HT29, HeLa, and Jurkat cell lysates; Human spleen tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-TRF2 antibody (Alexa Fluor® 488) [EPR3517(2)] (ab198595)
    Anti-TRF2 antibody (Alexa Fluor® 647) [EPR3517(2)] (ab198597)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
    • Tissue specificity
      Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
    • Sequence similarities
      Contains 1 HTH myb-type DNA-binding domain.
    • Domain
      The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
    • Information by UniProt
    • Database links
    • Alternative names
      • Telomeric DNA binding protein antibody
      • Telomeric DNA-binding protein antibody
      • Telomeric repeat binding factor 2 antibody
      • Telomeric repeat binding protein 2 antibody
      • Telomeric repeat-binding factor 2 antibody
      • TERF 2 antibody
      • Terf2 antibody
      • TERF2_HUMAN antibody
      • TRBF 2 antibody
      • TRBF2 antibody
      • TRF 2 antibody
      • TRF2 antibody
      • TTAGGG repeat binding factor 2 antibody
      • TTAGGG repeat-binding factor 2 antibody
      see all

    Images

    • All lanes : Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution

      Lane 1 : HT29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 56 kDa
    • ab108997 at 1/250 dilution staining TRF2 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
    • ab108997 at 1/100 dilution staining TRF2 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Kulak O  et al. Disruption of Wnt/ß-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells. Mol Cell Biol 35:2425-35 (2015). Read more (PubMed: 25939383) »
    • Tang M  et al. Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance. J Cell Sci 128:331-41 (2015). ICC/IF . Read more (PubMed: 25416818) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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