Overview

  • Product nameAnti-TRF2 antibody [EPR3518(2)]
    See all TRF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3518(2)] to TRF2
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human TRF2.

  • Positive control
    • Jurkat and NAMALWA cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionBinds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
    • Tissue specificityUbiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
    • Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
    • DomainThe TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
    • Information by UniProt
    • Database links
    • Alternative names
      • Telomeric DNA binding protein antibody
      • Telomeric DNA-binding protein antibody
      • Telomeric repeat binding factor 2 antibody
      • Telomeric repeat binding protein 2 antibody
      • Telomeric repeat-binding factor 2 antibody
      • TERF 2 antibody
      • Terf2 antibody
      • TERF2_HUMAN antibody
      • TRBF 2 antibody
      • TRBF2 antibody
      • TRF 2 antibody
      • TTAGGG repeat binding factor 2 antibody
      • TTAGGG repeat-binding factor 2 antibody
      see all

    Anti-TRF2 antibody [EPR3518(2)] images

    • All lanes : Anti-TRF2 antibody [EPR3518(2)] (ab108932) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : NAMALWA cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 56 kDa

    References for Anti-TRF2 antibody [EPR3518(2)] (ab108932)

    ab108932 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab108932.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"