Overview

  • Product name
    Anti-TRFP / MED20 antibody
  • Description
    Mouse polyclonal to TRFP / MED20
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length TRFP/ MED20 protein (Human)

  • Positive control
    • TRFP / MED20 transfected 293T cell lysate and Hela cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    NOTE: Product requires purification with Protein A prior to use in ICC/IF.

    WB: 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      TRFP / MED20 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
    • Cellular localization
      Nucleus
    • Database links
    • Alternative names
      • MED20 antibody
      • Mediator complex subunit 20 antibody
      • TRF proximal protein homolog antibody
      • TRFP antibody

    Images

    • All lanes : Anti-TRFP / MED20 antibody (ab67800) at 1/500 dilution

      Lane 1 : TRFP / MED20 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 23 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    • ab67800 at 10µg/ml staining TRFP/ MED20 in Hela cells.

    References

    ab67800 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab67800.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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