Overview

  • Product nameAnti-TRIAD3 antibody
    See all TRIAD3 primary antibodies
  • Description
    Rabbit polyclonal to TRIAD3
  • Specificityab25961 recognises TRIAD3A.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to 15 N terminal amino acids of Mouse TRIAD3

  • Positive control
    • Mouse heart cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab25961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).Can be blocked with Human TRIAD3 peptide (ab39886).

Target

  • RelevanceThe TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • CAHH antibody
    • E3 ubiquitin ligase TRIAD3 antibody
    • E3 ubiquitin-protein ligase RNF216 antibody
    • ring finger protein 216 antibody
    • RNF216 antibody
    • Triad domain-containing protein 3 antibody
    • TRIAD3 antibody
    • U7I1 antibody
    • UBCE7IP1 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 1 antibody
    • UiP83 antibody
    • ZIN antibody
    • Zinc finger protein inhibiting NF kappa B antibody
    see all

Anti-TRIAD3 antibody images

  • Lane 1 : Anti-TRIAD3 antibody (ab25961) at 0.5 µg/ml
    Lane 2 : Anti-TRIAD3 antibody (ab25961) at 1 µg/ml
    Lane 3 : Anti-TRIAD3 antibody (ab25961) at 2 µg/ml

    Lane 1 : Mouse heart cell lysates
    Lane 2 : Mouse heart cell lysates
    Lane 3 : Mouse heart cell lysates


    Predicted band size : 99 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • ab25961 at 10µg/ml staining TRIAD3 in mouse heart by IHC 

  • Immunofluorescence of TRIAD3A in Mouse Heart cells using ab25961 at 20 ug/ml.

References for Anti-TRIAD3 antibody (ab25961)

This product has been referenced in:
  • Rajagopalan S  et al. TNFR-associated factor 6 and TGF-ß-activated kinase 1 control signals for a senescence response by an endosomal NK cell receptor. J Immunol 192:714-21 (2014). Read more (PubMed: 24337384) »
  • Miah SM  et al. Ubiquitylation of an internalized killer cell Ig-like receptor by Triad3A disrupts sustained NF-?B signaling. J Immunol 186:2959-69 (2011). Read more (PubMed: 21270397) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam earlier today.

As we discussed on the telephone I am going to send you ab22058 to try out alongside ab25961 to see if the 130kDa band that you are seeing is the correct band or not. Your new order number is ****...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"