Anti-TRIAD3 antibody - N-terminal (ab189113)

Overview

  • Product name
    Anti-TRIAD3 antibody - N-terminal
    See all TRIAD3 primary antibodies
  • Description
    Rabbit polyclonal to TRIAD3 - N-terminal
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse TRIAD3 (N terminal).
    Database link: P58283

  • Positive control
    • Human testis tissue, mouse heart cells and mouse heart cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 97 kDa.

Target

  • Relevance
    The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • CAHH antibody
    • E3 ubiquitin ligase TRIAD3 antibody
    • E3 ubiquitin-protein ligase RNF216 antibody
    • ring finger protein 216 antibody
    • RNF216 antibody
    • Triad domain-containing protein 3 antibody
    • TRIAD3 antibody
    • U7I1 antibody
    • UBCE7IP1 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 1 antibody
    • UiP83 antibody
    • ZIN antibody
    • Zinc finger protein inhibiting NF kappa B antibody
    see all

Images

  • Lane 1 : Anti-TRIAD3 antibody - N-terminal (ab189113) at 0.5 µg/ml
    Lane 2 : Anti-TRIAD3 antibody - N-terminal (ab189113) at 1 µg/ml
    Lane 3 : Anti-TRIAD3 antibody - N-terminal (ab189113) at 2 µg/ml

    Lane 1 : mouse heart cell lysate
    Lane 2 : mouse heart cell lysate
    Lane 3 : mouse heart cell lysate


    Predicted band size : 97 kDa
  • Immunohistochemical analysis of paraffin embedded formalin fixed human testis tissue labeling TRIAD3 using ab189113 at 5 µg/ml.

  • Immunofluorescent analysis of mouse heart cells labeling TRIAD3 with ab189113 at 20 ug/ml.

References

ab189113 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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