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Rabbit polyclonal to TRIB1
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Saccharomyces cerevisiae
Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (EERTQLRLES LEDTHIMKGE DDALSDKHGC PAYVSPEILN TTGTYSGKAA) of Human TRIB1 (NP_079471).
Human fetal intestine lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.
Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain.
The protein kinase domain is predicted to be catalytically inactive.
Information by UniProt
G-protein-coupled receptor-induced gene 2 protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB1 antibody (ab89021)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling TRIB1 with ab89021 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I and II cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TRIB1. Right - Merge.
Western blot - Anti-TRIB1 antibody (ab89021)
Western blot analysis of COLO205 cell lysate labeling TRIB1 with ab89021 at 1.0µg/ml.
Western blot - TRIB1 antibody (ab89021)
Anti-TRIB1 antibody (ab89021) at 1 µg/ml + Human fetal intestine lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa Gel concentration: 12%
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"