Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIB1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (EERTQLRLES LEDTHIMKGE DDALSDKHGC PAYVSPEILN TTGTYSGKAA) of Human TRIB1 (NP_079471).

  • Positive control
    • Human fetal intestine lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.
  • Tissue specificity
    Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • Database links
  • Alternative names
    • C8FW antibody
    • G-protein-coupled receptor-induced gene 2 protein antibody
    • GIG-2 antibody
    • GIG2 antibody
    • SKIP1 antibody
    • TRB-1 antibody
    • TRB1 antibody
    • Trib1 antibody
    • TRIB1_HUMAN antibody
    • Tribbles homolog 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling TRIB1 with ab89021 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I and II cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TRIB1. Right - Merge.


  • Predicted band size : 41 kDa
    Western blot analysis of COLO205 cell lysate labeling TRIB1 with ab89021 at 1.0µg/ml.
  • Anti-TRIB1 antibody (ab89021) at 1 µg/ml + Human fetal intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Gel concentration: 12%

References

This product has been referenced in:
  • Makishima S  et al. Sin3A-associated protein, 18 kDa, a novel binding partner of TRIB1, regulates MTTP expression. J Lipid Res 56:1145-52 (2015). Read more (PubMed: 25921304) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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