Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIB2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (YPFHDIEPSS LFSKIRRGQF NIPETLSPKA KCLIRSILRR EPSERLTSQE) of Human TRIB2 (NP_067675).

  • Positive control
    • Human fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-TRIB2 antibody (ab84683) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Xu S  et al. TRIB2 inhibits Wnt/ß-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases, ß-TrCP, COP1 and Smurf1, in liver cancer cells. FEBS Lett 588:4334-41 (2014). Read more (PubMed: 25311538) »
  • Qiao Y  et al. Ubiquitin E3 ligase SCF(ß-TRCP) regulates TRIB2 stability in liver cancer cells. Biochem Biophys Res Commun 441:555-9 (2013). WB ; Human . Read more (PubMed: 24211582) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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