FunctionDisrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Tissue specificityHighest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily. Contains 1 protein kinase domain.
All lanes : Anti-TRIB3 antibody (ab73547) at 1/1000 dilution
Lane 1 : Lysate prepared from rat soleus tissue, homogenized in TPER Lane 2 : Lysate prepared from rat soleus tissue, homogenized in TPER Lane 3 : Lysate prepared from rat liver tissue, homogenized in TPER
Lysates/proteins at 60 µg per lane.
Secondary rabbit monoclonal conjugated to HRP at 1/20000 dilution Developed using the ECL technique
Rzymski T et al. Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. Oncogene27:4532-43 (2008).
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