- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TRIB3 antibody
See all TRIB3 primary antibodies -
DescriptionRabbit polyclonal to TRIB3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Fr, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide from the N-terminal domain of human TRIB3
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: Whole serum -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab73547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 40 kDa. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionDisrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
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Tissue specificityHighest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
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Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 57761 Human
- Entrez Gene: 228775 Mouse
- Entrez Gene: 246273 Rat
- Omim: 607898 Human
- SwissProt: Q96RU7 Human
- SwissProt: Q8K4K2 Mouse
- SwissProt: Q9WTQ6 Rat
- Unigene: 516826 Human
see all -
Alternative names
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
see all
Images
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Western blot - TRIB3 antibody (ab73547)Anti-TRIB3 antibody (ab73547) at 1/500 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 38 kDa (why is the actual band size different from the predicted?)
Additional bands at: 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
Western blot - TRIB3 antibody (ab73547)Image courtesy of an anonymous Abreview.All lanes : Anti-TRIB3 antibody (ab73547) at 1/1000 dilution
Lanes 1-2 : Lysate prepared from rat soleus tissue, homogenized in TPER
Lane 3 : Lysate prepared from rat liver tissue, homogenized in TPER
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : rabbit monoclonal conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?)
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
References
This product has been referenced in:
- Rzymski T et al. Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. Oncogene 27:4532-43 (2008). WB ; Human . Read more (PubMed: 18408768) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"