Customer reviews and FAQs

Overview

  • Product name
    Anti-TRIB3 antibody
    See all TRIB3 primary antibodies
  • Description
    Rabbit polyclonal to TRIB3
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from the N-terminal domain of human TRIB3

Properties

Applications

Our Abpromise guarantee covers the use of ab73547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
  • Tissue specificity
    Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • C20orf97 antibody
    • Neuronal cell death inducible putative kinase antibody
    • Neuronal cell death-inducible putative kinase antibody
    • NIPK antibody
    • p65 interacting inhibitor of NF-kappaB antibody
    • p65-interacting inhibitor of NF-kappa-B antibody
    • SINK antibody
    • SKIP 3 antibody
    • SKIP3 antibody
    • TRB 3 antibody
    • TRB-3 antibody
    • TRB3 antibody
    • TRIB 3 antibody
    • Trib3 antibody
    • TRIB3_HUMAN antibody
    • Tribbles homolog 3 antibody
    • Tribbles pseudokinase 3 antibody
    • Tribbles3 antibody
    see all

Anti-TRIB3 antibody images

  • Western blot - TRIB3 antibody (ab73547)
    Western blot - TRIB3 antibody (ab73547)
    Anti-TRIB3 antibody (ab73547) at 1/500 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Western blot - TRIB3 antibody (ab73547)
    Western blot - TRIB3 antibody (ab73547)
    All lanes : Anti-TRIB3 antibody (ab73547) at 1/1000 dilution

    Lane 1 : Lysate prepared from rat soleus tissue, homogenized in TPER
    Lane 2 : Lysate prepared from rat soleus tissue, homogenized in TPER
    Lane 3 : Lysate prepared from rat liver tissue, homogenized in TPER

    Lysates/proteins at 60 µg per lane.

    Secondary
    rabbit monoclonal conjugated to HRP at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-TRIB3 antibody (ab73547)

This product has been referenced in:
  • Rzymski T  et al. Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. Oncogene 27:4532-43 (2008). WB ; Human . Read more (PubMed: 18408768) »

See 1 Publication for this product

Publishing research using ab73547? Please let us know so that we can cite the reference in this datasheet.

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2 Abreviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-TRIB3 antibody

 Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neural stem cell)
Permeabilization
Yes - 0.2% Triton-X
Specification
neural stem cell
Blocking step
Serum as blocking agent for 3 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
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Abcam user community

Verified customer

Submitted Apr 19 2016

Western blot abreview for Anti-TRIB3 antibody

 Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (soleus and liver)
Loading amount
60 µg
Specification
soleus and liver
Treatment
Homogenized in TPER (Pierce) Buffer
Gel Running Conditions
Reduced Denaturing (7% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
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Abcam user community

Verified customer

Submitted Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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