Anti-TRIB3 antibody - Aminoterminal end (ab50516)

Overview

  • Product nameAnti-TRIB3 antibody - Aminoterminal end
    See all TRIB3 primary antibodies
  • Description
    Rabbit polyclonal to TRIB3 - Aminoterminal end
  • SpecificityThis antibody is specific to TRIB3. The immunizing sequence has no identity to TRIB1 and TRIB2 isoforms.
  • Tested applicationsSuitable for: WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    GSLSRKKRLELDDNLDTER

    , corresponding to N terminal amino acids 11-29 of Human TRIB3 conjugated to KLH

  • Positive control
    • HEK-293 cells expressing human TRB3.

Properties

Applications

Our Abpromise guarantee covers the use of ab50516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. Staining of the TRB3 band is specifically inhibited with the immunizing peptide.
IHC-Fr 1/200.
IP Use at an assay dependent concentration.

Target

  • FunctionDisrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
  • Tissue specificityHighest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf97 antibody
    • Neuronal cell death inducible putative kinase antibody
    • Neuronal cell death-inducible putative kinase antibody
    • NIPK antibody
    • p65 interacting inhibitor of NF-kappaB antibody
    • p65-interacting inhibitor of NF-kappa-B antibody
    • SINK antibody
    • SKIP 3 antibody
    • SKIP3 antibody
    • TRB 3 antibody
    • TRB-3 antibody
    • TRB3 antibody
    • TRIB 3 antibody
    • Trib3 antibody
    • TRIB3_HUMAN antibody
    • Tribbles homolog 3 antibody
    • Tribbles pseudokinase 3 antibody
    • Tribbles3 antibody
    see all

Anti-TRIB3 antibody - Aminoterminal end images

  • All lanes : Anti-TRIB3 antibody - Aminoterminal end (ab50516) at 1/1000 dilution

    Lane 1 : HEK293 cells transfected with human TRIB3
    Lane 2 : HEK293 cells (untransfected)
    Lane 3 : HEK293 cells transfected with human TRIB3 with TRIB3 immunizing peptide at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size : 40 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ab50516 staining TRIB3 in human brain tumor tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized 0.5% TX-100 before blocking with 10% serum for 1 hour 30 minutes at 250C. The sample was incubated with primary antibody (1/200) in 1% serum in PBS for 2 hours at 250C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

References for Anti-TRIB3 antibody - Aminoterminal end (ab50516)

This product has been referenced in:
  • Salazar M  et al. Cannabinoid action induces autophagy-mediated cell death through stimulation of ER stress in human glioma cells. J Clin Invest 119:1359-72 (2009). IP ; Human . Read more (PubMed: 19425170) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC in a PT link
Permeabilization No
Specification Muscle
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 24 2015

Thank you for contacting us.  Both of these anti-TRIB3 antibodies has been tested for immunoprecipiation and are guaranteed for its use in this application. They have not been tested side by side and so we have no data on whether one may work better fo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela cells)
Total protein in input 500 µg
Specification Hela cells
Treatment cannabinoids (THC) for 8h
Immuno-precipitation step Protein G
Username

Ms. María Salazar Roa

Verified customer

Submitted Aug 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (tumor xenografts from human glioma cells)
Specification tumor xenografts from human glioma cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX-100
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Mar Lorente

Verified customer

Submitted Jul 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"