Recombinant Anti-TRIB3 antibody [EPR3150] (ab75774)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3150] to TRIB3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TRIB3 antibody [EPR3150]
See all TRIB3 primary antibodies -
Description
Rabbit monoclonal [EPR3150] to TRIB3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TRIB3 aa 50-150. The exact sequence is proprietary.
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Positive control
- 293T lysates, untreated or treated with Ionomycin and TPA. WB: HeLa cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3150 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. -
Tissue specificity
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57761 Human
- Omim: 607898 Human
- SwissProt: Q96RU7 Human
- Unigene: 516826 Human
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Alternative names
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
see all
Images
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All lanes : Anti-TRIB3 antibody [EPR3150] (ab75774) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRIB3 knockout HeLa cell lysate
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDaFalse colour image of Western blot: Anti-TRIB3 antibody [EPR3150] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab75774 was shown to bind specifically to TRIB3. A band was observed at 40 kDa in wild-type HeLa cell lysates with no signal observed at this size in TRIB3 knockout cell line ab264792 (knockout cell lysate ab257765). To generate this image, wild-type and TRIB3 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-TRIB3 antibody [EPR3150] (ab75774) at 1/1000 dilution
Lane 1 : 293T lysates, treated with Ionomycin and
TPA
Lane 2 : 293T lysates, untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab75774 has not yet been referenced specifically in any publications.