The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 220 kDa.
Use at an assay dependent dilution. PubMed: 19786578
Use at an assay dependent dilution. PubMed: 19038788
FunctionIntermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
Tissue specificityFound in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium.
Sequence similaritiesBelongs to the S100-fused protein family. Contains 2 EF-hand domains.
Developmental stageExpressed during late differentiation of the epidermis.
DomainConsists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species.
Post-translational modificationsSubstrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase.
Western blot - Anti-Trichohyalin antibody [AE15] (ab58755)
Anti-Trichohyalin antibody [AE15] (ab58755) at 1/100 dilution + Lung (Human) Tissue Lysate at 10 µg
Secondary Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 220 kDa Observed band size : 220 kDa Additional bands at : 165 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - Anti-Trichohyalin antibody [AE15] (ab58755)This image is courtesy of an anonymous Abreview
ab58755 staining Trichohyalin in mouse skin tissue sections by Immunohistochemistry (IHC-Fr - paraformaldehyde-fixed, frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with a mouse blocking reagent for 1 hour at 25°C. The sample was incubated with primary antibody (1/100 in Diluent) at 4°C for 12 hours. An Alexa Fluor®546-conjugated Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trichohyalin antibody [AE15] (ab58755)This image is courtesy of an anonymous Abreview
ab58755 staining Trichohyalin in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.
References for Anti-Trichohyalin antibody [AE15] (ab58755)
This product has been referenced in:
Yuan S et al. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22. PLoS Genet11:e1005253 (2015).
Read more (PubMed: 26020521) »
Rognoni E et al. Kindlin-1 controls Wnt and TGF-ß availability to regulate cutaneous stem cell proliferation. Nat Med20:350-9 (2014).
Read more (PubMed: 24681597) »