Anti-TRIF antibody (ab13810)
Key features and details
- Rabbit polyclonal to TRIF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TRIF antibody
See all TRIF primary antibodies -
Description
Rabbit polyclonal to TRIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Mouse TRIF aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q80UF7 -
Positive control
- WB: Mouse spleen, intestine and RAW 264.7 cell lysates. IHC-P: Mouse spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 79.23 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
IHC-P |
1/200.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 79.23 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
IHC-P
1/200. |
Target
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Function
Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. -
Tissue specificity
Ubiquitously expressed but with higher levels in liver. -
Sequence similarities
Contains 1 TIR domain. -
Domain
The N-terminal region is essential for activation of the IFNB promoter activity. -
Post-translational
modificationsPhosphorylated by TBK1. - Information by UniProt
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Database links
- Entrez Gene: 106759 Mouse
- Entrez Gene: 363328 Rat
- SwissProt: Q80UF7 Mouse
- Unigene: 203952 Mouse
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Alternative names
- IIAE6 antibody
- MGC35334 antibody
- MyD88 3 antibody
see all
Images
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All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse spleen lysate with immunizing peptide
Lane 2 : Mouse spleen lysate
Predicted band size: 79.23 kDa -
Immunohistochemistrical analysis of a FFPE tissue section of mouse spleen using 1/200 dilution of ab13810. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse intestine lysate
Lane 2 : Mouse spleen lysate
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Predicted band size: 79.23 kDa
Protocols
Datasheets and documents
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Datasheet download
References (46)
ab13810 has been referenced in 46 publications.
- Kim YK et al. Differential susceptibility to lipopolysaccharide affects the activation of toll-like-receptor 4 signaling in THP-1 cells and PMA-differentiated THP-1 cells. Innate Immun 28:122-129 (2022). PubMed: 35612375
- Muendlein HI et al. ZBP1 promotes inflammatory responses downstream of TLR3/TLR4 via timely delivery of RIPK1 to TRIF. Proc Natl Acad Sci U S A 119:e2113872119 (2022). PubMed: 35666872
- Hornigold K et al. Age-related decline in the resistance of mice to bacterial infection and in LPS/TLR4 pathway-dependent neutrophil responses. Front Immunol 13:888415 (2022). PubMed: 36090969
- Liu Y et al. Antiviral activity of portulaca oleracea L. extracts against porcine epidemic diarrhea virus by partial suppression on myd88/NF-?b activation in vitro. Microb Pathog 154:104832 (2021). PubMed: 33781871
- Nihon-Yanagi Y et al. Immunohistochemical Analysis of Toll-Like Receptors, MyD88, and TRIF in Human Papillary Thyroid Carcinoma and Anaplastic Thyroid Carcinoma. J Thyroid Res 2021:4226491 (2021). PubMed: 34306609