Overview

  • Product nameAnti-TRIF antibody
    See all TRIF primary antibodies
  • Description
    Rabbit polyclonal to TRIF
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to 14 amino acids near the C-terminus of human TRIF.

  • Positive control
    • Human lung cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab62583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionInvolved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
  • Tissue specificityUbiquitously expressed but with higher levels in liver.
  • Sequence similaritiesContains 1 TIR domain.
  • DomainThe N-terminal region is essential for activation of the IFNB promoter activity.
  • Post-translational
    modifications
    Phosphorylated by TBK1.
  • Information by UniProt
  • Database links
  • Alternative names
    • IIAE6 antibody
    • MGC35334 antibody
    • MyD88 3 antibody
    • Proline rich vinculin and TIR domain containing protein B antibody
    • Proline-rich antibody
    • PRVTIRB antibody
    • Putative NF kappa B activating protein 502H antibody
    • Putative NF-kappa-B-activating protein 502H antibody
    • Putative NFkB activating protein antibody
    • TCAM1_HUMAN antibody
    • TICAM 1 antibody
    • TICAM-1 antibody
    • Ticam1 antibody
    • TIR domain containing adapter molecule 1 antibody
    • TIR domain containing adapter protein inducing IFN beta antibody
    • TIR domain containing adaptor inducing interferon beta antibody
    • TIR domain-containing adapter molecule 1 antibody
    • TIR domain-containing adapter protein inducing IFN-beta antibody
    • Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody
    • Toll like receptor adaptor molecule 1 antibody
    • Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody
    • TRIF antibody
    • TRIF protein antibody
    • vinculin and TIR domain-containing protein B antibody
    see all

Anti-TRIF antibody images

  • Lane 1 : Anti-TRIF antibody (ab62583) at 2 µg/ml
    Lane 2 : Anti-TRIF antibody (ab62583) at 4 µg/ml

    Lane 1 : Human lung cell lysates
    Lane 2 : Human lung cell lysates

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 76 kDa
    Observed band size : 76 kDa
  • Immunohistochemical analysis of TRIF expression in paraffin-embedded human lung tissue using ab62583 at 10 µg/ml.
  • Immunofluorescence of TRIF in Human Lung cells using ab62583 at 10 ug/ml.

References for Anti-TRIF antibody (ab62583)

This product has been referenced in:
  • Banerjee S  et al. TLR signaling-mediated differential histone modification at IL-10 and IL-12 promoter region leads to functional impairments in tumor-associated macrophages. Carcinogenesis : (2011). WB ; Human . Read more (PubMed: 21926109) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (macrophages)
Loading amount 25000 cells
Specification macrophages
Gel Running Conditions Reduced Denaturing (NuPAGE 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted May 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"