Overview

  • Product name
    Triglyceride Assay Kit - Quantification 
    See all Triglyceride kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    > 2 µM
  • Range
    2 µM - 10000 µM
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammal
  • Product overview

    Triglyceride Quantification Assay Kit (ab65336) provides a sensitive, easy assay to measure triglyceride (TG) concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product which reacts with the probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).


    If your sample contains reducing substances such as glucose, fructose or lactose, they are likely to interfere with the assay. In this case, we recommend to use PicoProbe Triglyceride Quantification Assay Kit (Fluorometric) (ab178780).



    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This kit also detects monoglycerides and diglycerides.

    Some components of this kit are available for purchasing as standalone reagents if you need more quantities (lipase, enzyme mix, TG standard or TG probe). Please contact our team for more details.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Relevance
    Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
  • Alternative names
    • Triacylglyceride

Associated products

Applications

Our Abpromise guarantee covers the use of ab65336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Triglyceride Assay Kit - Quantification  images

  • Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.

    HepG2 cells were treated with 25 uM Chloroquine for 72h.

  • Colorimetric triglyceride standard curve using ab65336.

Protocols

References for Triglyceride Assay Kit - Quantification  (ab65336)

This product has been referenced in:
  • Kanuri BN  et al. Altered glucose and lipid homeostasis in liver and adipose tissue pre-dispose inducible NOS knockout mice to insulin resistance. Sci Rep 7:41009 (2017). Functional Studies ; Mouse . Read more (PubMed: 28106120) »
  • Yin F  et al. Transcriptome Analysis Reveals Regulation of Gene Expression for Lipid Catabolism in Young Broilers by Butyrate Glycerides. PLoS One 11:e0160751 (2016). Functional Studies ; Chicken . Read more (PubMed: 27508934) »

See all 40 Publications for this product

Product Wall

1) The insoluble top layer may contain the triglyceride you wish to measure so please make sure to solubilize as much as possible (without losing any material) and to follow the recommendations (ie. repeated heating steps).
2) SV is indeed the abb...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I contacted the laboratories and they recommended to use IGEPAL® detergent at <=0.5%.

I hope this will be helpful and I am happy to answer any further...

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Comparing a serial dilution of yellow-bellied marmot (Marmota flaviventris) to a standard serial dilution, we determined the quantity of plasma to use in subsequent assays (shown only the greatest dilution fell on the curve).
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Abcam user community

Verified customer

Submitted Jul 22 2016

The picoprobe allows ab178780 to be more sensitive than the ab65336. ab178780 detects triglycerides <0.4uM whereas ab65336 can detect 2uM-10mM triglycerides.

I hope this helps!



The lab has recommended to use more than 1 ml NP-40/water solution to process the fattier tissue. You can also heat for >5 mins to make sure that all the TG has been extracted. For the colorimetric assay, having fat globules in the sample ...

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1. This solution is only water+ 5%NP-40. (example: 5 ul NP-40 and 95 ul water).

2. If there is 15 mg tissue, try using less volume of water+NP-40. Whether you use neat or diluted sample will depend on the TG content of the sample. ...

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We do not suggest extracting triglyceride and cholesterol in an unique extraction step with two layers. We have not tested this with each kit and cannot guarantee the effectiveness. Triglyceride is simply solubilized in detergent-water and total l...

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I can confirm that both EDTA or Heparin can be used for plasma sample preparation.

In order to avoid any interference because of protein matrix effect, we would advise to try different dilutions e.g. 1/10 to /50.

The assay can be applied to tissue culture medium samples. We recommend testing a range of sample volumes per well, for instance 2, 10, and 50ul per well. Adjust all the volumes to 50ul with the Triglyceride Assay Buffer. Avoid phenol red if you can, t...

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The lipase is from a bacterial source and the standards are from plant oils.

1-10 of 40 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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