Frozen storage may cause the triglyceride standard to separate
from the aqueous phase. To re-dissolve, keep the cap tightly closed, place in a hot water bath (~80-100°C) for 1 minute or until the standard looks cloudy, vortex for 30 seconds, the standard should become clear. Repeat the heat and vortex one more time. The triglyceride standard is now completely in solution, and ready to use.
RelevanceTriglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.