Triiodothyronine (T3) ELISA Kit (ab108685)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameTriiodothyronine (T3) ELISA Kit
See all Triiodothyronine (T3) kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 40 < 10.7% Inter-assay Sample n Mean SD CV% Overall 3 < 9.1% -
Sample typeSerum, Plasma
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Assay typeCompetitive
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Sensitivity0.05 ng/ml
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Recovery
97.5 %
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.
A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.
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Tested applicationsSuitable for: Competitive ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 11X T3-HRP Conjugate 1 x 1.4ml 50X Wash Solution 1 x 20ml Anti-T3 IgG Coated Microplate (12 x 8 wells) 1 unit Conjugate Buffer 1 x 12.5ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit T3 Standard 0 - 0.0 ng/mL 1 x 1ml T3 Standard 1 - 0.5 ng/mL 1 x 1ml T3 Standard 2 - 1.0 ng/mL 1 x 1ml T3 Standard 3 - 2.5 ng/mL 1 x 1ml T3 Standard 4 - 5.0 ng/mL 1 x 1ml T3 Standard 5 - 7.5 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
Research areas
Applications
Our Abpromise guarantee covers the use of ab108685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent dilution. |
Triiodothyronine (T3) ELISA Kit images
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Typical Standard CurveRepresentative Standard Curve using ab108685
Protocols
References for Triiodothyronine (T3) ELISA Kit (ab108685)
ab108685 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"