Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.
A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|11X T3-HRP Conjugate||1 x 1.4ml|
|50X Wash Solution||1 x 20ml|
|Anti-T3 IgG Coated Microplate (12 x 8 wells)||1 unit|
|Conjugate Buffer||1 x 12.5ml|
|Cover foils||1 unit|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|T3 Standard 0 - 0.0 ng/mL||1 x 1ml|
|T3 Standard 1 - 0.5 ng/mL||1 x 1ml|
|T3 Standard 2 - 1.0 ng/mL||1 x 1ml|
|T3 Standard 3 - 2.5 ng/mL||1 x 1ml|
|T3 Standard 4 - 5.0 ng/mL||1 x 1ml|
|T3 Standard 5 - 7.5 ng/mL||1 x 1ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|