Triiodothyronine (T3) ELISA Kit (ab108685)

Overview

• Product nameTriiodothyronine (T3) ELISA Kit
  See all Triiodothyronine (T3) kits
• Detection methodColorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Overall	40			< 10.7%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Overall	3			< 9.1%

• Sample type
  Serum, Plasma
• Assay typeCompetitive
• Sensitivity
  0.05 ng/ml
• Recovery

  97.5 %
  

• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.

  
  

   

  
  

  A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

• Tested applicationsSuitable for: Competitive ELISAmore details
• PlatformMicroplate

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  11X T3-HRP Conjugate	1 x 1.4ml
  50X Wash Solution	1 x 20ml
  Anti-T3 IgG Coated Microplate (12 x 8 wells)	1 unit
  Conjugate Buffer	1 x 12.5ml
  Cover foils	1 unit
  Stop Solution	1 x 15ml
  Strip holder	1 unit
  T3 Standard 0 - 0.0 ng/mL	1 x 1ml
  T3 Standard 1 - 0.5 ng/mL	1 x 1ml
  T3 Standard 2 - 1.0 ng/mL	1 x 1ml
  T3 Standard 3 - 2.5 ng/mL	1 x 1ml
  T3 Standard 4 - 5.0 ng/mL	1 x 1ml
  T3 Standard 5 - 7.5 ng/mL	1 x 1ml
  TMB Substrate Solution	1 x 15ml

• Research areas
  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Growth factors and hormones ELISA kits

Triiodothyronine (T3) ELISA Kit images

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  Typical Standard Curve

  Representative Standard Curve using ab108685