Overview

  • Product name
    Triiodothyronine (T3) ELISA Kit
    See all Triiodothyronine (T3) kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 40 < 10.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 < 9.1%
  • Sample type
    Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    0.05 ng/ml
  • Recovery

    97.5 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.


     


    A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    11X T3-HRP Conjugate 1 x 1.4ml
    50X Wash Solution 1 x 20ml
    Anti-T3 IgG Coated Microplate (12 x 8 wells) 1 unit
    Conjugate Buffer 1 x 12.5ml
    Cover foils 1 unit
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    T3 Standard 0 - 0.0 ng/mL 1 x 1ml
    T3 Standard 1 - 0.5 ng/mL 1 x 1ml
    T3 Standard 2 - 1.0 ng/mL 1 x 1ml
    T3 Standard 3 - 2.5 ng/mL 1 x 1ml
    T3 Standard 4 - 5.0 ng/mL 1 x 1ml
    T3 Standard 5 - 7.5 ng/mL 1 x 1ml
    TMB Substrate Solution 1 x 15ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108685

Protocols

References

This product has been referenced in:
  • Gil-Cayuela C  et al. Myocardium of patients with dilated cardiomyopathy presents altered expression of genes involved in thyroid hormone biosynthesis. PLoS One 13:e0190987 (2018). Read more (PubMed: 29320567) »

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