Anti-TRIM10 antibody (ab151306)
Key features and details
- Rabbit polyclonal to TRIM10
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM10 antibody -
Description
Rabbit polyclonal to TRIM10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 26-265 of Human TRIM10 (Uniprot ID: Q9UDY6)
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Positive control
- NT2D1 cells lysate; IMR32, U87-MG and MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
TRIM10 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic bodies. Studies in mice suggest that this protein plays a role in terminal differentiation of erythroid cells. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 10107 Human
- Omim: 605701 Human
- SwissProt: Q9UDY6 Human
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Alternative names
- B30 RING finger protein antibody
- Hematopoietic RING finger 1 antibody
- HERF1 antibody
see all
Images
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Immunofluorescent analysis of methanol-fixed MCF7 cells labeling TRIM10 with ab151306 antibody at 1/200 dilution. Bottom figure represents cells co-stained with Hoechst 33342.
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Anti-TRIM10 antibody (ab151306) at 1/1000 dilution + NT2D1 whole cells lysate at 30 µg
Predicted band size: 55 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151306 has been referenced in 2 publications.
- Yang H et al. Tripartite motif 10 regulates cardiac hypertrophy by targeting the PTEN/AKT pathway. J Cell Mol Med 24:6233-6241 (2020). PubMed: 32343488
- Kimura T et al. Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy. EMBO J 36:42-60 (2017). PubMed: 27932448