Overview

  • Product nameAnti-TRIM14 antibody
    See all TRIM14 primary antibodies
  • Description
    Rabbit polyclonal to TRIM14
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: TWLKGKFTEL RLLLDEEEAL AKKFIDKNTQ LTLQVYREQA DSCREQLDIM, corresponding to N terminal amino acids 109-158 of Human TRIM14

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab50941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

  • FunctionInhibits the transcriptional activity of SPI1 in a dose-dependent manner.
  • Tissue specificityHighest expression in liver; undetectable in skeletal muscle.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
  • DomainThe B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5830400N10Rik antibody
    • KIAA0129 antibody
    • pub antibody
    • TRI14_HUMAN antibody
    • TRIM14 antibody
    • tripartite motif protein 14 antibody
    • tripartite motif protein TRIM14 antibody
    • tripartite motif-containing 14 antibody
    • Tripartite motif-containing protein 14 antibody
    see all

Anti-TRIM14 antibody images

  • Anti-TRIM14 antibody (ab50941) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)

References for Anti-TRIM14 antibody (ab50941)

ab50941 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"