The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay play a role in the regulation of keratinocyte differentiation.
Tissue specificityHighest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
Sequence similaritiesBelongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain.
ab111734 at 1/50 dilution staining TRIM16 in paraffin-embedded Human Testis tissue by Immunohistochemistry. Left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.
References for Anti-TRIM16 antibody (ab111734)
has not yet been referenced specifically in any publications.
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