Overview

  • Product nameAnti-TRIM16 antibody
    See all TRIM16 primary antibodies
  • Description
    Rabbit polyclonal to TRIM16
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human TRIM16, NP_006461.3.

  • Positive control
    • Whole cell lysate from Hela, 293T and NIH 3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay play a role in the regulation of keratinocyte differentiation.
  • Tissue specificityHighest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EBBP antibody
    • Estrogen responsive B box protein antibody
    • Estrogen-responsive B box protein antibody
    • TRI16_HUMAN antibody
    • TRIM 16 antibody
    • TRIM16 antibody
    • Tripartite motif containing 16 antibody
    • Tripartite motif containing protein 16 antibody
    • Tripartite motif-containing protein 16 antibody
    see all

Anti-TRIM16 antibody images

  • All lanes : Anti-TRIM16 antibody (ab72129) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of TRIM16.
    Lane 1: ab72129 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72129 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 10 seconds.

References for Anti-TRIM16 antibody (ab72129)

ab72129 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"