• Product nameAnti-TRIM21 antibody
    See all TRIM21 primary antibodies
  • Description
    Rabbit polyclonal to TRIM21
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TRIM21 aa 12-262 (internal sequence).
    Database link: BC010861

  • Positive control
    • PG49, HeLa, MCF7, HEK293 cell lysates and Human fetal spleen lysate; Human fetal small intestine tissue.



Our Abpromise guarantee covers the use of ab119895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 45, 54 kDa).
IHC-P 1/100 - 1/500.


  • FunctionE3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2.
  • Tissue specificityIsoforms 1 and 2 are expressed in fetal and adult heart and fetal lung.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • DomainThe coiled-coil is necessary for the cytoplasmic localization. The B30.2/SPRY domain is necessary for the cytoplasmic localization, the interaction with IRF3 and for the IRF3-driven interferon beta promoter activity. The RING-type zinc finger is necessary for ubiquitination and for the IRF3-driven interferon beta promoter activity. Interacts with SKP2 and CUL1 in a RING finger-independent manner.
  • Post-translational
    Autoubiquitinated; does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > P-body. Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • 52 kDa ribonucleoprotein autoantigen Ro/SS-A antibody
    • 52 kDa Ro protein antibody
    • 52kD Ro/SSA autoantigen antibody
    • Autoantigen Ro/SSA, 52-KD antibody
    • E3 ubiquitin-protein ligase TRIM21 antibody
    • RING finger protein 81 antibody
    • RNF81 antibody
    • Ro 52 antibody
    • Ro(SS-A) antibody
    • Ro52 antibody
    • RO52_HUMAN antibody
    • Sicca syndrome antigen A antibody
    • Sjoegren syndrome type A antigen antibody
    • Sjogren syndrome antigen A1 antibody
    • Sjogren syndrome type A antigen antibody
    • SS-A antibody
    • SSA antibody
    • SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro) antibody
    • TRIM21 antibody
    • Tripartite motif protein TRIM21 antibody
    • Tripartite motif-containing 21 antibody
    • Tripartite motif-containing protein 21 antibody
    see all

Anti-TRIM21 antibody images

  • All lanes : Anti-TRIM21 antibody (ab119895) at 1/500 dilution

    Lane 1 : PG49 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HEK293 cell lysate
    Lane 5 : Human fetal spleen lysate

    Predicted band size : 45, 54 kDa
  • ab119895 at 1/100 dilution staining SSA1 in Formalin-fixed, Paraffin-embedded Human fetal small intestine tissue by Immunohistochemistry.

References for Anti-TRIM21 antibody (ab119895)

ab119895 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 24 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (Bone marrow derived macrophages)
Specification Bone marrow derived macrophages
Treatment crude LPS 600ng/ml for 16 hours and untreated
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Melissa Wallin

Verified customer

Submitted May 12 2014