Overview

  • Product nameAnti-Trim22 antibody
    See all Trim22 primary antibodies
  • Description
    Mouse polyclonal to Trim22
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-498 of Human Trim22 (isoform 1)

  • Positive control
    • Trim22 transfected mammalian cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).

Target

  • RelevanceTrim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms.
  • Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Nucleus; Cajal body. Note: Localizes predominanltly into the nucleus, found in cytoplasm to some extent. Forms distinct nuclear bodies that undergo dynamic changes during cell cycle progression. Nuclear bodies start to form in the early G0/G1 phase but become speckle-like in the S-phase and completely dispersed in mitosis. 35% of TRIM22 nuclear bodies overlap or are found adjacent to Cajal bodies.
  • Database links
  • Alternative names
    • 50 kDa stimulated trans acting factor antibody
    • GPSTAF50 antibody
    • RING finger protein 94 antibody
    • RNF94 antibody
    • Staf 50 antibody
    • STAF50 antibody
    • Stimulated trans acting factor (50 kDa) antibody
    • Stimulated Trans Acting Factor (homolog of Mouse Rpt 1 gene) antibody
    • Trim22 antibody
    • Tripartite binding motif 22 antibody
    • Tripartite motif containing protein 22 antibody
    • TTRIM22RIM22 antibody
    see all

Anti-Trim22 antibody images

  • All lanes : Anti-Trim22 antibody (ab68071) at 1/500 dilution

    Lane 1 : Trim22 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-Trim22 antibody (ab68071)

ab68071 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"