Overview

  • Product nameAnti-Trim22 antibody
    See all Trim22 primary antibodies
  • Description
    Rabbit polyclonal to Trim22
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide from within a region of internal sequence amino acids 359-408 (DVSGKIAWIL GVHSKISSLN KRKSSGFAFD PSVNYSKVYS RYRPQYGYWV) of Human Trim22 (NP_006065)

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceTrim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms.
  • Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Nucleus; Cajal body. Note: Localizes predominanltly into the nucleus, found in cytoplasm to some extent. Forms distinct nuclear bodies that undergo dynamic changes during cell cycle progression. Nuclear bodies start to form in the early G0/G1 phase but become speckle-like in the S-phase and completely dispersed in mitosis. 35% of TRIM22 nuclear bodies overlap or are found adjacent to Cajal bodies.
  • Database links
  • Alternative names
    • 50 kDa stimulated trans acting factor antibody
    • GPSTAF50 antibody
    • RING finger protein 94 antibody
    • RNF94 antibody
    • Staf 50 antibody
    • STAF50 antibody
    • Stimulated trans acting factor (50 kDa) antibody
    • Stimulated Trans Acting Factor (homolog of Mouse Rpt 1 gene) antibody
    • Trim22 antibody
    • Tripartite binding motif 22 antibody
    • Tripartite motif containing protein 22 antibody
    • TTRIM22RIM22 antibody
    see all

Anti-Trim22 antibody images

  • Anti-Trim22 antibody (ab86116) at 1 µg/ml (5% skim milk/ PBS buffer) + trabsfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Trim22 antibody (ab86116)

ab86116 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"