Anti-TRIM26 antibody (ab89290)
Key features and details
- Mouse polyclonal to TRIM26
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM26 antibody
See all TRIM26 primary antibodies -
Description
Mouse polyclonal to TRIM26 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TRIM26. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- A-431 cell lysate and TRIM26 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. |
Target
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Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. - Information by UniProt
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Database links
- Entrez Gene: 7726 Human
- Omim: 600830 Human
- SwissProt: Q12899 Human
- Unigene: 485041 Human
- Unigene: 731863 Human
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Alternative names
- Acid finger protein antibody
- AFP antibody
- CAM25547 antibody
see all
Images
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Anti-TRIM26 antibody (ab89290) at 1 µg/ml + A-431 cell lysate at 50 µg
Predicted band size: 62 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-TRIM26 antibody (ab89290) at 1 µg/ml
Lane 1 : TRIM26 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 62 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (4)
ab89290 has been referenced in 4 publications.
- Li X et al. Deubiquitinase USP39 and E3 ligase TRIM26 balance the level of ZEB1 ubiquitination and thereby determine the progression of hepatocellular carcinoma. Cell Death Differ 28:2315-2332 (2021). PubMed: 33649471
- Williams SC & Parsons JL NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress. Mol Cell Biol 38:N/A (2018). PubMed: 29610152
- Edmonds MJ et al. Ubiquitylation-dependent regulation of NEIL1 by Mule and TRIM26 is required for the cellular DNA damage response. Nucleic Acids Res 45:726-738 (2017). PubMed: 27924031
- Zhang X et al. Spina bifida in fetus is associated with an altered pattern of DNA methylation in placenta. J Hum Genet N/A:N/A (2015). PubMed: 26178427