• Product nameAnti-TRIM27 antibody
    See all TRIM27 primary antibodies
  • Description
    Rabbit polyclonal to TRIM27
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A synthetic peptide corresponding to a region of Human TRIM27

  • Positive control
    • Fetal muscle lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab76587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.


  • FunctionHas a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA (By similarity). E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation.
  • Tissue specificityExpressed in testis namely within the seminiferous tubules.
  • Involvement in diseaseDefects in TRIM27 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • DomainThe coiled-coil region mediates interaction with EPC1 and CHD4. The B box and coiled-coil domains mediate interaction with PML. The B box and the distal coiled-coil domains mediate homomultimerisation. The B30.2 domain mediates interaction with EIF3S6.
  • Cellular localizationNucleus. Cytoplasm. Nucleus > PML body. Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW538890 antibody
    • DAAP-182E11.3 antibody
    • MGC189472 antibody
    • OTTHUMP00000029314 antibody
    • Ret finger protein antibody
    • RFP antibody
    • RFP transforming protein antibody
    • Rfp1 antibody
    • RING finger protein 76 antibody
    • RNF76 antibody
    • TRI27_HUMAN antibody
    • TRIM 27 antibody
    • Trim27 antibody
    • Tripartite motif containing 27 antibody
    • Tripartite motif containing protein 27 antibody
    • Tripartite motif protein TRIM27 antibody
    • Tripartite motif-containing protein 27 antibody
    • Zinc finger protein RFP antibody
    see all

Anti-TRIM27 antibody images

  • Anti-TRIM27 antibody (ab76587) at 1 µg + Fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TRIM27 antibody (ab76587)

ab76587 has not yet been referenced specifically in any publications.

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