Overview

  • Product nameAnti-TRIM29 antibody
    See all TRIM29 primary antibodies
  • Description
    Rabbit polyclonal to TRIM29
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 538 and 588 of human TRIM29 (NP_036233.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab74193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionIt is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
    • Tissue specificityExpressed in placenta, prostate and thymus.
    • Sequence similaritiesContains 1 B box-type zinc finger.
    • Post-translational
      modifications
      Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
    • Cellular localizationCytoplasm. Colocalizes with intermediate filaments.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ataxia telangiectasia group D associated protein antibody
      • Ataxia telangiectasia group D-associated protein antibody
      • ATDC antibody
      • FLJ36085 antibody
      • TRI29_HUMAN antibody
      • TRIM 29 antibody
      • TRIM29 antibody
      • Tripartite motif containing 29 antibody
      • Tripartite motif containing protein 29 antibody
      • Tripartite motif protein 29 antibody
      • Tripartite motif protein TRIM29 antibody
      • Tripartite motif-containing protein 29 antibody
      see all

    Anti-TRIM29 antibody images

    • Detection of TRIM29 by Western Blot of Immunprecipitate.
      ab74193 at 1µg/ml staining TRIM29 in HeLa whole cell lysate immunoprecipitated using ab74193 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.
    • IHC image of ab74193 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74193, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-TRIM29 antibody (ab74193)

    ab74193 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"