The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa). An approximately 70 kDa band was observed in Human Peripheral Blood Mononucleocytes lysates. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.
FunctionIt is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
Tissue specificityExpressed in placenta, prostate and thymus.
Sequence similaritiesContains 1 B box-type zinc finger.
Post-translational modificationsConstitutively phosphorylated by PKC on serine/threonine in A431 cells.
Cellular localizationCytoplasm. Colocalizes with intermediate filaments.