Overview

  • Product nameAnti-TRIM29 antibody
    See all TRIM29 primary antibodies
  • Description
    Goat polyclonal to TRIM29
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-EKDRIKSFTTNE

    , corresponding to internal sequence amino acids 295-306 of Human TRIM29 (NP_036233.2).

  • Positive control
    • Human Peripheral Blood Mononucleocytes lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab99486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa). An approximately 70 kDa band was observed in Human Peripheral Blood Mononucleocytes lysates. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Target

  • FunctionIt is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
  • Tissue specificityExpressed in placenta, prostate and thymus.
  • Sequence similaritiesContains 1 B box-type zinc finger.
  • Post-translational
    modifications
    Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
  • Cellular localizationCytoplasm. Colocalizes with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ataxia telangiectasia group D associated protein antibody
    • Ataxia telangiectasia group D-associated protein antibody
    • ATDC antibody
    • FLJ36085 antibody
    • TRI29_HUMAN antibody
    • TRIM 29 antibody
    • TRIM29 antibody
    • Tripartite motif containing 29 antibody
    • Tripartite motif containing protein 29 antibody
    • Tripartite motif protein 29 antibody
    • Tripartite motif protein TRIM29 antibody
    • Tripartite motif-containing protein 29 antibody
    see all

Anti-TRIM29 antibody images

  • Anti-TRIM29 antibody (ab99486) at 1 µg/ml + Human Peripheral Blood Lymphocytes lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 66 kDa
  • ab99486 (3.8µg/ml) staining of paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-TRIM29 antibody (ab99486)

ab99486 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"