Overview

  • Product nameAnti-TRIM3 antibody
  • Description
    Rabbit polyclonal to TRIM3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from N terminal residues of Human TRIM3 protein (NP_006449).

  • Positive control
    • Human fetal heart, Human fetal brain, 293T, RAW264.7 and LoVo lysates.

Properties

  • FormLyophilised:Reconstitute in 200ul Sterile Distilled Water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, 1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111840 is purified by a peptide affinity column.
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab111840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 81 kDa.
IHC-P 1/100 - 1/500.

Target

  • RelevanceTRIM3 a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM3 localizes to cytoplasmic filaments and is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified.
  • Cellular localizationCytoplasmic; Early endosome
  • Database links
  • Alternative names
    • BERP antibody
    • Brain expressed RING finger protein antibody
    • FLJ16135 antibody
    • HAC1 antibody
    • Ring finger protein 22 antibody
    • RNF22 antibody
    • RNF9 antibody
    • Tripartite motif containing 3 antibody
    see all

Anti-TRIM3 antibody images

  • All lanes : Anti-TRIM3 antibody (ab111840) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : 293T lysate
    Lane 4 : RAW264.7 lysate
    Lane 5 : LoVo lysate


    Observed band size : 81 kDa (why is the actual band size different from the predicted?)

References for Anti-TRIM3 antibody (ab111840)

ab111840 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"