Overview

  • Product nameAnti-TRIM35 antibody
    See all TRIM35 primary antibodies
  • Description
    Rabbit polyclonal to TRIM35
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to a region within the C terminal amino acids 144-193 (FRVRSLIAEE RRNFLPTHQW IVTKTRLQTS SPNLQSRRQG QVQEHGACTA) of Human TRIM35 (NP_741983).

  • Positive control
    • Human fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57, 60 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/1562500.

Target

  • FunctionMay play a role as a tumor suppressor. Implicated in the cell death mechanism.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • DomainThe RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity.
  • Cellular localizationCytoplasm. Nucleus. Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0710005M05Rik antibody
    • A430106H13Rik antibody
    • AW046487 antibody
    • Hemopoietic lineage switch protein 5 antibody
    • HLS5 antibody
    • KIAA1098 antibody
    • Macrophage derived apoptosis inducing RBCC protein antibody
    • MAIR antibody
    • MGC17233 antibody
    • mKIAA1098 antibody
    • NC8 antibody
    • Protein MAIR antibody
    • Protein Nc8 antibody
    • TRI35_HUMAN antibody
    • TRIM35 antibody
    • Tripartite motif containing 35 antibody
    • Tripartite motif containing protein 35 antibody
    • Tripartite motif-containing protein 35 antibody
    see all

Anti-TRIM35 antibody images

  • Anti-TRIM35 antibody (ab83243) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57,60 kDa (why is the actual band size different from the predicted?)

References for Anti-TRIM35 antibody (ab83243)

ab83243 has not yet been referenced specifically in any publications.

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