Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM35
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 180-229 (AAWLEGRIRQ EFDKLREFLR VEEQAILDAM AEETRQKQLL ADEKMKQLTE)of human TRIM35 (NP_741983).

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May play a role as a tumor suppressor. Implicated in the cell death mechanism.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity.
  • Cellular localization
    Cytoplasm. Nucleus. Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0710005M05Rik antibody
    • A430106H13Rik antibody
    • AW046487 antibody
    • Hemopoietic lineage switch protein 5 antibody
    • HLS5 antibody
    • KIAA1098 antibody
    • Macrophage derived apoptosis inducing RBCC protein antibody
    • MAIR antibody
    • MGC17233 antibody
    • mKIAA1098 antibody
    • NC8 antibody
    • Protein MAIR antibody
    • Protein Nc8 antibody
    • TRI35_HUMAN antibody
    • TRIM35 antibody
    • Tripartite motif containing 35 antibody
    • Tripartite motif containing protein 35 antibody
    • Tripartite motif-containing protein 35 antibody
    see all

Images

  • Anti-TRIM35 antibody (ab87169) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 24 kDa,60 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling TRIM35 with ab87169 at 5.0µg/ml.

References

ab87169 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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