Overview

  • Product nameAnti-TRIM41 antibody
    See all TRIM41 primary antibodies
  • Description
    Rabbit polyclonal to TRIM41
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 35-84 (CGHNFCRVCV TQLWGGEDEE DRDELDREEE EEDGEEEEVE AVGAGAGWDT) of Human TRIM41 (NP_291027).

  • Positive control
    • Human fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IP Use at an assay dependent concentration.
ICC/IF 1/200.

Target

  • FunctionFunctions as an E3 ligase that catalyzes the ubiquitin-mediated degradation of protein kinase C.
  • Tissue specificityExpressed in multiple tissues with the highest levels in heart and skeletal muscle.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase TRIM41 antibody
    • MGC1127 antibody
    • MGC31991 antibody
    • RINCK antibody
    • RING finger protein that interacts with C kinase antibody
    • RING finger-interacting protein with C kinase antibody
    • TRI41_HUMAN antibody
    • Trim41 antibody
    • Tripartite motif containing 41 antibody
    • Tripartite motif-containing protein 41 antibody
    see all

Anti-TRIM41 antibody images

  • Anti-TRIM41 antibody (ab98170) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 72 kDa
  • ab98170 staining TRIM41 in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.5% and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG H+L polyclonal(1/500) was used as the secondary antibody.

    See Abreview

References for Anti-TRIM41 antibody (ab98170)

ab98170 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HepG2.2.15)
Total protein in input 5000 µg
Specification HepG2.2.15
Treatment siRNA transfected
Immuno-precipitation step Protein A/G
Username

Dr. Shijian Zhang

Verified customer

Submitted Mar 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2)
Specification HepG2
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton-X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Shijian Zhang

Verified customer

Submitted Mar 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 10000 cells
Specification HepG2
Treatment transfected with TRIM41 expression plasmid
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Shijian Zhang

Verified customer

Submitted Mar 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"