Overview

  • Product name
    Anti-TRIM42 antibody
  • Description
    Rabbit polyclonal to TRIM42
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab60335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

Images

  • All lanes : Anti-TRIM42 antibody (ab60335) at 0.5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Armistead PM  et al. Common minor histocompatibility antigen discovery based upon patient clinical outcomes and genomic data. PLoS One 6:e23217 (2011). WB ; Human . Read more (PubMed: 21858034) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab60335.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up