Anti-TRIM45 antibody (ab169036)
Key features and details
- Mouse polyclonal to TRIM45
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM45 antibody -
Description
Mouse polyclonal to TRIM45 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant full length protein corresponding to Human TRIM45 aa 1-562. Isoform 2
Sequence:MSENRKPLLGFVSKLTSGTALGNSGKTHCPLCLGLFKAPRLLPCLHTVCT TCLEQLEPFSVVDIRGGDSDTSSEGSIFQELKPRSLQSQIGILCPVCDAQ VDLPMGGVKALTIDHLAVNDVMLESLRGEGQGLVCDLCNDREVEKRCQTC KANLCHFCCQAHRRQKKTTYHTMVDLKDLKGYSRIGKPILCPVHPAEELR LFCEFCDRPVCQDCVVGEHREHPCDFTSNVIHKHGDSVWELLKGTQPHVE ALEEALAQIHIINSALQKRVEAVAADVRTFSEGYIKAIEEHRDKLLKQLE DIRAQKENSLQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKRVV VERLRKLNKVQYSTRPGVNDKIRFCPQEKAGQCRGYEIYDLHRAREKQTA SFTLLCKDAAGEIMGRGGDNVQVAVVPKDKKDSPVRTMVQDNKDGTYYIS YTPKEPGVYTVWVCIKEQHVQGSPFTVTVRRKHRPHSGVFHCCTFCSSGG QKTARCACGGTMPGGYLGCGHGHKGHPGHPHWSCCGKFNEKSECTWTGGQ SAPRSLLRTVAL
Database link: AAH34943.1 -
Positive control
- TRIM45-transfected 293T cell line lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. -
Tissue specificity
Expressed in skeletal muscle, brain, heart and pancreas. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 filamin repeat.
Contains 1 RING-type zinc finger. -
Developmental stage
Expressed in brain, lung, skeletal muscle, heart and intestine in 80-day old embryo. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 80263 Human
- Omim: 609318 Human
- SwissProt: Q9H8W5 Human
- Unigene: 301526 Human
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Alternative names
- FLJ13181 antibody
- RING finger protein 99 antibody
- RNF99 antibody
see all
Images
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Immunofluorescent analysis of permeabilized HeLa cells labeling TRIM45 with ab169036 at 10µg/ml.
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All lanes : Anti-TRIM45 antibody (ab169036) at 1 µg/ml
Lane 1 : TRIM45-transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab169036 has not yet been referenced specifically in any publications.