Overview

  • Product name
  • Description
    Mouse polyclonal to TRIM45
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant full length protein corresponding to Human TRIM45 aa 1-562. Isoform 2
    Sequence:

    MSENRKPLLGFVSKLTSGTALGNSGKTHCPLCLGLFKAPRLLPCLHTVCT TCLEQLEPFSVVDIRGGDSDTSSEGSIFQELKPRSLQSQIGILCPVCDAQ VDLPMGGVKALTIDHLAVNDVMLESLRGEGQGLVCDLCNDREVEKRCQTC KANLCHFCCQAHRRQKKTTYHTMVDLKDLKGYSRIGKPILCPVHPAEELR LFCEFCDRPVCQDCVVGEHREHPCDFTSNVIHKHGDSVWELLKGTQPHVE ALEEALAQIHIINSALQKRVEAVAADVRTFSEGYIKAIEEHRDKLLKQLE DIRAQKENSLQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKRVV VERLRKLNKVQYSTRPGVNDKIRFCPQEKAGQCRGYEIYDLHRAREKQTA SFTLLCKDAAGEIMGRGGDNVQVAVVPKDKKDSPVRTMVQDNKDGTYYIS YTPKEPGVYTVWVCIKEQHVQGSPFTVTVRRKHRPHSGVFHCCTFCSSGG QKTARCACGGTMPGGYLGCGHGHKGHPGHPHWSCCGKFNEKSECTWTGGQ SAPRSLLRTVAL


    Database link: AAH34943.1

  • Positive control
    • TRIM45-transfected 293T cell line lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab169036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.
  • Tissue specificity
    Expressed in skeletal muscle, brain, heart and pancreas.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 1 RING-type zinc finger.
  • Developmental stage
    Expressed in brain, lung, skeletal muscle, heart and intestine in 80-day old embryo.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ13181 antibody
    • RING finger protein 99 antibody
    • RNF99 antibody
    • TRI45_HUMAN antibody
    • TRIM45 antibody
    • Tripartite motif containing 45 antibody
    • Tripartite motif containing protein 45 antibody
    • Tripartite motif-containing protein 45 antibody
    see all

Images

  • Immunofluorescent analysis of permeabilized HeLa cells labeling TRIM45 with ab169036 at 10µg/ml.

  • All lanes : Anti-TRIM45 antibody (ab169036) at 1 µg/ml

    Lane 1 : TRIM45-transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa

References

ab169036 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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