Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM45
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within residues: FKAPRLLPCL HTVCTTCLEQ LEPFSVVDIR GGDSDTSSEG SIFQELKPRS , corresponding to N terminal amino acids 36-85 of Human TRIM45 (NP_079464)

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.
  • Tissue specificity
    Expressed in skeletal muscle, brain, heart and pancreas.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 1 RING-type zinc finger.
  • Developmental stage
    Expressed in brain, lung, skeletal muscle, heart and intestine in 80-day old embryo.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ13181 antibody
    • RING finger protein 99 antibody
    • RNF99 antibody
    • TRI45_HUMAN antibody
    • TRIM45 antibody
    • Tripartite motif containing 45 antibody
    • Tripartite motif containing protein 45 antibody
    • Tripartite motif-containing protein 45 antibody
    see all

Images

  • Anti-TRIM45 antibody (ab84303) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa

References

This product has been referenced in:
  • Sato T  et al. The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway. Oncogene 0:N/A (2014). Read more (PubMed: 24681954) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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