Anti-TRIM47 antibody (ab72234)
Key features and details
- Rabbit polyclonal to TRIM47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM47 antibody -
Description
Rabbit polyclonal to TRIM47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human TRIM47.
Database link: Q96LD4 -
Positive control
- Whole cell lysate from Hela and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 70, 80, 130 kDa (predicted molecular weight: 70 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 70, 80, 130 kDa (predicted molecular weight: 70 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
TRIM47 belongs to the TRIM/RBCC family. It contains one B box type zinc finger, one B30.2/SPRY domain and one RING type zinc finger. TRIM47 has low expression in most tissues; higher expression in kidney tubular cells and over expressed in astrocytoma tumor cells. -
Cellular localization
Cytoplasm. Nucleus -
Database links
- Entrez Gene: 738407 Chimpanzee
- Entrez Gene: 618638 Cow
- Entrez Gene: 91107 Human
- Omim: 611041 Human
- SwissProt: Q96LD4 Human
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Alternative names
- Gene overexpressed in astrocytoma protein antibody
- GOA antibody
- RING finger protein 100 antibody
see all
Images
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All lanes : Anti-TRIM47 antibody (ab72234) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 70 kDa
Observed band size: 70,80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation/ Western Blot of TRIM47.
Lane 1: ab72234 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72234 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72234 has not yet been referenced specifically in any publications.