Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM47
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide from a region between residue 125 and 175 of human TRIM47, NP_258411.2.

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 70 and 80 and 130 kDa (predicted molecular weight: 70 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      TRIM47 belongs to the TRIM/RBCC family. It contains one B box type zinc finger, one B30.2/SPRY domain and one RING type zinc finger. TRIM47 has low expression in most tissues; higher expression in kidney tubular cells and over expressed in astrocytoma tumor cells.
    • Cellular localization
      Cytoplasm. Nucleus
    • Database links
    • Alternative names
      • Gene overexpressed in astrocytoma protein antibody
      • GOA antibody
      • RING finger protein 100 antibody
      • RNF100 antibody
      • Tripartite motif containing 47 antibody
      • Tripartite motif containing protein 47 antibody
      see all

    Images

    • All lanes : Anti-TRIM47 antibody (ab72234) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 70 kDa
      Observed band size : 70,80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of TRIM47.
      Lane 1: ab72234 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72234 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 3 seconds.

    References

    ab72234 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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