Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM5 alpha
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRIM5 alpha (internal sequence). The exact sequence is proprietary. NM_033034.2.
    Database link: Q9C035

  • Positive control
    • Human brain cortex tissue: Human stomach cells: Human stomach, thymus and uterus cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab190235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 56 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • Post-translational
    modifications
    Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
  • Cellular localization
    Cytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • RING finger protein 88 antibody
    • RNF88 antibody
    • TRIM5 antibody
    • TRIM5_HUMAN antibody
    • TRIM5alpha antibody
    • Tripartite motif containing 5 antibody
    • tripartite motif protein TRIM5 antibody
    • Tripartite motif-containing protein 5 antibody
    see all

Images

  • All lanes : Anti-TRIM5 alpha antibody (ab190235) at 2 µg/ml

    Lane 1 : Human stomach cell lysate
    Lane 2 : Human thymus cell lysate
    Lane 3 : Human uterus cell lysates


    Predicted band size : 56 kDa
  • Immunofluorescent analysis of Human stomach cells labeling TRIM5 alpha with ab190235 at 10 µg/ml. 

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling TRIM5 alpha with ab190235 at 2 µg/ml.

References

ab190235 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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