Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM5 alpha
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    A synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha

  • Positive control
    • Human uterus, thymus and stomach lysates and human breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function
    Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • Post-translational
    modifications
    Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
  • Cellular localization
    Cytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • RING finger protein 88 antibody
    • RNF88 antibody
    • TRIM5 antibody
    • TRIM5_HUMAN antibody
    • TRIM5alpha antibody
    • Tripartite motif containing 5 antibody
    • tripartite motif protein TRIM5 antibody
    • Tripartite motif-containing protein 5 antibody
    see all

Anti-TRIM5 alpha antibody images

  • All lanes : Anti-TRIM5 alpha antibody (ab59000) at 2 µg/ml

    Lane 1 : human stomach cell lysate
    Lane 2 : human thymus cell lysate
    Lane 3 : human uterus cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab59000 at 10ug/ml staining TRIM5 alpha in human breast by Immunohistochemistry, PFA-fixed tissue (4%).

References for Anti-TRIM5 alpha antibody (ab59000)

ab59000 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (FRhK cell)
Specification
FRhK cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
3% BSA plus 2% goat serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2010

Application
Western blot
Sample
Monkey Cell lysate - whole cell (FRhK4 cell line)
Loading amount
25 µg
Specification
FRhK4 cell line
Gel Running Conditions
Reduced Denaturing (15% TrisHCl Criterion gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Dec 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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