Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM5 gamma
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to 15 amino acids near the carboxy-terminus of Human TRIM5 gamma.

  • Positive control
    • Human Small Intestine and Human Pancreas tissue. Human bladder and colon cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • Post-translational
    modifications
    Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
  • Cellular localization
    Cytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • RING finger protein 88 antibody
    • RNF88 antibody
    • TRIM5 antibody
    • TRIM5_HUMAN antibody
    • Tripartite motif containing 5 antibody
    • Tripartite motif protein TRIM5 antibody
    • Tripartite motif-containing protein 5 antibody
    see all

Images

  • All lanes : Anti-TRIM5 gamma antibody (ab109763) at 2 µg/ml

    Lane 1 : Human bladder lysate
    Lane 2 : Human colon lysate


    Predicted band size : 40 kDa
  • ab109763, at 2.5 µg/ml, staining TRIM5 gamma in formalin-fixed, paraffin-embedded Human Small Intestine tissue by immunohistochemistry. Sections were incubated with a biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109763, at 2.5 µg/ml, staining TRIM5 gamma in formalin-fixed, paraffin-embedded Human pancreas tissue by immunohistochemistry. Sections were incubated with a biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

ab109763 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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