Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM56
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding a region between residues 425 and 475 of human TRIM56 (NP_112223.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab74194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked polyubiquitination of TMEM173/STING, thereby playing a key role in innate immunity. TMEM173/STING 'Lys-63'-linked ubiquitination activates the production of type I interferon IFN-beta following detection of pathogen- and host-derived double-stranded DNA.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A130009K11Rik antibody
    • DKFZp667O116 antibody
    • E3 ubiquitin-protein ligase TRIM56 antibody
    • FLJ35608 antibody
    • Gm452 antibody
    • MGC37358 antibody
    • OTTMUSP00000027392 antibody
    • RING finger protein 109 antibody
    • RNF109 antibody
    • TRI56_HUMAN antibody
    • Trim56 antibody
    • Tripartite motif containing protein 56 antibody
    • Tripartite motif-containing protein 56 antibody
    see all

Images

  • All lanes : Anti-TRIM56 antibody (ab74194) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 81 kDa
    Observed band size : 81 kDa


    Exposure time : 3 minutes
  • Detection of Human TRIM56 by Western Blot of Immunprecipitate.
    All lanes: ab74194, at 1µg/ml, staining TRIM56 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
    Lane 1: ab74194
    Lane 2: Control IgG.
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab74194 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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