Validated using a knockout cell line

Anti-TRIM56 antibody [EPR10582] (ab154821)


  • Product name
    Anti-TRIM56 antibody [EPR10582]
    See all TRIM56 primary antibodies
  • Description
    Rabbit monoclonal [EPR10582] to TRIM56
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human TRIM56 (Q9BRZ2).

  • Positive control
    • MCF-7, HeLa and A375 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab154821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 81 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked polyubiquitination of TMEM173/STING, thereby playing a key role in innate immunity. TMEM173/STING 'Lys-63'-linked ubiquitination activates the production of type I interferon IFN-beta following detection of pathogen- and host-derived double-stranded DNA.
    • Sequence similarities
      Belongs to the TRIM/RBCC family.
      Contains 2 B box-type zinc fingers.
      Contains 1 RING-type zinc finger.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • A130009K11Rik antibody
      • DKFZp667O116 antibody
      • E3 ubiquitin-protein ligase TRIM56 antibody
      • FLJ35608 antibody
      • Gm452 antibody
      • MGC37358 antibody
      • OTTMUSP00000027392 antibody
      • RING finger protein 109 antibody
      • RNF109 antibody
      • TRI56_HUMAN antibody
      • Trim56 antibody
      • Tripartite motif containing protein 56 antibody
      • Tripartite motif-containing protein 56 antibody
      see all


    • ab154821 staining TRIM56 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab154821 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TRIM56 knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: A375 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab154821 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab154821 was shown to specifically react with TRIM56 when TRIM56 knockout samples were used. Wild-type and TRIM56 knockout samples were subjected to SDS-PAGE. ab154821 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling TRIM56  with purified ab154821 at 1:50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/1000 dilution + MCF-7 (human breast carcinoma) whole cell lysates at 10 µg

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 81 kDa
      Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.

    • Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/5000 dilution + A549 (human lung carcinoma) whole cell lysates at 20 µg

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 81 kDa
      Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.

    • All lanes : Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/1000 dilution (unpurified)

      Lane 1 : MCF 7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 81 kDa


    ab154821 has not yet been referenced specifically in any publications.

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