Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM68
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (IRLRKGNEYR AGTDEYPILS LPVPPRRVGI FVDYEAHDIS FYNVTDCGSH) of human TRIM68 (NP_060543).

  • Positive control
    • HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.
  • Tissue specificity
    Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING domain is essential for ubiquitin E3 ligase activity.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localization
    Cytoplasm > perinuclear region. Nucleus. Colocalized with AR in nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase TRIM68 antibody
    • FLJ10369 antibody
    • GC109 antibody
    • MGC126176 antibody
    • RING finger protein 137 antibody
    • RNF 137 antibody
    • RNF137 antibody
    • Ro/SSA1 related protein antibody
    • SS 56 antibody
    • SS-56 antibody
    • SS56 antibody
    • SSA protein SS 56 antibody
    • SSA protein SS-56 antibody
    • TRI68_HUMAN antibody
    • TRIM 68 antibody
    • Trim68 antibody
    • Tripartite motif containing 68 antibody
    • Tripartite motif containing protein 68 antibody
    • Tripartite motif-containing protein 68 antibody
    see all

Images

  • Anti-TRIM68 antibody (ab86393) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 36 kDa,42 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TRIM68 with ab86393 at 5.0µg/ml.

References

ab86393 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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