Overview

  • Product nameAnti-TRIM71 antibody
    See all TRIM71 primary antibodies
  • Description
    Rabbit polyclonal to TRIM71
  • Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide (15 amino acid) from near the C terminus of Human TRIM71 (NP_001034200).

  • Positive control
    • Human brain tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab105330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
ICC Use a concentration of 5 µg/ml.

Target

  • FunctionE3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
  • Tissue specificitySpecifically expressed in testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Autoubiquitinated.
  • Cellular localizationCytoplasm, P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610206G21Rik antibody
    • Abnormal cell LINeage LIN 41 antibody
    • AL022943 antibody
    • E3 ubiquitin-protein ligase TRIM71 antibody
    • Gm1127 antibody
    • Homolog of C. elegans Lin 41 antibody
    • LIN 41 antibody
    • LIN41 antibody
    • LIN41_HUMAN antibody
    • mlin 41 antibody
    • Protein lin 41 homolog antibody
    • Protein lin-41 homolog antibody
    • RGD1566388 antibody
    • Ripply2 antibody
    • TRIM 71 antibody
    • TRIM71 antibody
    • Tripartite motif containing 71 antibody
    • Tripartite motif-containing protein 71 antibody
    see all

Anti-TRIM71 antibody images

  • Lane 1 : Anti-TRIM71 antibody (ab105330) at 1 µg/ml
    Lane 2 : Anti-TRIM71 antibody (ab105330) at 2 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti-rabbit secondary antibody

    Predicted band size : 93 kDa
  • Immunohistochemistry of TRIM71 in human brain tissue with ab105330 at 5 µg/ml.
  • Immunofluorescence of TRIM71 in Human Brain cells using ab105330 at 20 ug/ml.

References for Anti-TRIM71 antibody (ab105330)

ab105330 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (human embryonic stem cells)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 50 µg
Specification human embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Miguel Fidalgo

Verified customer

Submitted Nov 10 2015

Abreviews
Application Western blot
Sample Human Tissue lysate - whole (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"