Overview

  • Product nameAnti-TRIM71 antibody
    See all TRIM71 primary antibodies
  • Description
    Goat polyclonal to TRIM71
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids of Human TRIM71.

  • Positive control
    • Human Brain Cerebellum tissue; HEK293 and HEK293 lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab117607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 93 kDa.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • FunctionE3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
  • Tissue specificitySpecifically expressed in testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Autoubiquitinated.
  • Cellular localizationCytoplasm, P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610206G21Rik antibody
    • Abnormal cell LINeage LIN 41 antibody
    • AL022943 antibody
    • E3 ubiquitin-protein ligase TRIM71 antibody
    • Gm1127 antibody
    • Homolog of C. elegans Lin 41 antibody
    • LIN 41 antibody
    • LIN41 antibody
    • LIN41_HUMAN antibody
    • mlin 41 antibody
    • Protein lin 41 homolog antibody
    • Protein lin-41 homolog antibody
    • RGD1566388 antibody
    • Ripply2 antibody
    • TRIM 71 antibody
    • TRIM71 antibody
    • Tripartite motif containing 71 antibody
    • Tripartite motif-containing protein 71 antibody
    see all

Anti-TRIM71 antibody images

  • ab117607, at 3.75 µg/ml, staining TRIM71 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry.
  • Anti-TRIM71 antibody (ab117607) at 0.5 µg/ml + HEK293 lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 93 kDa

References for Anti-TRIM71 antibody (ab117607)

ab117607 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Zebrafish Tissue lysate - whole (2 day embryos)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (7% gel)
Loading amount 75 µg
Specification 2 day embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Mar 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"