The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa). An approximately 100 kDa band was observed in lysates of the cell line NIH3T3. An additional band of unknown identity was also consistently observed at 14 kDa. This band was successfully blocked by incubation with the immunizing peptide.
FunctionE3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
Tissue specificitySpecifically expressed in testis.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 filamin repeat. Contains 6 NHL repeats. Contains 1 RING-type zinc finger.