Overview

  • Product nameAnti-TRIM72 antibody
    See all TRIM72 primary antibodies
  • Description
    Mouse polyclonal to TRIM72
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-269 of Human TRIM72 (isoform 2)

  • Positive control
    • Liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 53 , 30 kDa.

Target

  • FunctionMuscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
  • Cellular localizationCell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOC493829 antibody
    • Mg53 antibody
    • Mitsugumin-53 antibody
    • TRI72_HUMAN antibody
    • trim72 antibody
    • Tripartite motif containing 72 antibody
    • Tripartite motif containing protein 72 antibody
    • Tripartite motif-containing protein 72 antibody
    see all

Anti-TRIM72 antibody images

  • Anti-TRIM72 antibody (ab68061) at 1/500 dilution + Human liver tissue lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

    Predicted band size : 53 , 30 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TRIM72 antibody (ab68061) at 1/500 dilution

    Lane 1 : TRIM72 (aa1-269, isoform 2) transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 53 , 30 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-TRIM72 antibody (ab68061)

ab68061 has not yet been referenced specifically in any publications.

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