Anti-TRIM72 antibody - C-terminal (ab170540)

Overview

  • Product name
    Anti-TRIM72 antibody - C-terminal
    See all TRIM72 primary antibodies
  • Description
    Rabbit polyclonal to TRIM72 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRIM72 aa 306-335 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6ZMU5

  • Positive control
    • NCI-H292 cell line lysate; Human pancreas tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170540 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 53 kDa.

Target

  • Function
    Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
  • Cellular localization
    Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOC493829 antibody
    • Mg53 antibody
    • Mitsugumin-53 antibody
    • TRI72_HUMAN antibody
    • trim72 antibody
    • Tripartite motif containing 72 antibody
    • Tripartite motif containing protein 72 antibody
    • Tripartite motif-containing protein 72 antibody
    see all

Images

  • Anti-TRIM72 antibody - C-terminal (ab170540) at 1/100 dilution + NCI-H292 cell line lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 53 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human pancreas tissue labeling TRIM72 with ab170540 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB detection.

References

ab170540 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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