Overview

  • Product nameAnti-TRIM9 antibody
    See all TRIM9 primary antibodies
  • Description
    Rabbit polyclonal to TRIM9
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 500-549 (DGLHFNSTYN ARVKAFNKTG VSPYSKTLVL QTSEGKALQQ YPSERELRGI) of Human TRIM9 (NP_443210).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

  • RelevanceTRIM9 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase TRIM9 antibody
    • HGNC:16288 antibody
    • Homolog of rat RING finger Spring antibody
    • KIAA0282 antibody
    • RING finger protein 91 antibody
    • RNF 91 antibody
    • RNF91 antibody
    • SPRING antibody
    • TRIM 9 antibody
    • Tripartite motif containing 9 antibody
    • Tripartite motif containing protein 9 antibody
    • Tripartite motif protein 9 antibody
    see all

Anti-TRIM9 antibody images

  • Anti-TRIM9 antibody (ab82654) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 79 kDa
    Observed band size : 61 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,37 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TRIM9 antibody (ab82654)

ab82654 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"